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KMID : 0380620110430060689
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Korean Journal of Food Science and Technology
2011 Volume.43 No. 6 p.689 ~ p.695
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Optimization of Lipase-Catalyzed Interesterification for Production of Human Milk Fat Substitutes by Response Surface Methodology
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Son Jeoung-Mae
Lee Jeung-Hee
Xue Cheng-Lian
Hong Soon-Tack
Lee Ki-Teak
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Abstract
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1,3-Dioleoyl-2-palmitoylglycerol (OPO)-rich human milk fat substitute (HMFS) was synthesized from tripalmitin (PPP)-rich fraction and oleic ethyl ester by a lipase-catalyzed interesterification. Response surface methodology (RSM) was employed to optimize the presence of palmitic acid at sn-2 position (Y1, %) and of oleic acid at sn-1,3 (Y2, %), with the reaction factors as substrate molar ratio of PPP-rich fraction to oleic ethyl ester (X1, 1:4, 1:5 and 1:6), reaction temperature (X2, 50, 55 and 60oC), and time (X3, 3, 7.5 and 12 h). The optimal conditions for HMFS synthesis were predicted at the reaction combination of 55oC, 3 h and 1:6 substrate ratio. HMFS re-synthesized under the same conditions displayed 70.70% palmitic acid at the sn-2 position and 69.58% oleic acid at the sn-1,3 position. Reaction product was predominantly (90.35%) triacylglycerol (TAG) was observed in which the major TAG species, OPO, comprised 31.24%.
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KEYWORD
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human milk fat substrate (HMFS), tripalmitin, oleic ethyl ester, response surface methodology
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